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LV200

Bioluminescence imaging system

The LV200 has been carefully designed for long-duration cell bioluminescence microscopy and is compatible with a broad range of cameras and objectives. A completely new optical design dramatically increases sensitivity and enables the detailed study of photosensitive cells and luminescence probes at high magnification. An inbuilt system for temperature control, humidity and gas flow helps to keep the cultured cells or tissue slices in a healthy condition throughout the observation period and the unique light-tight enclosure shields the sample and optics from any external light.

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Completely new optical design for greater sensitivity

Conventional microscope frames are optimised for transmission and fluorescence applications and are not sensitive enough to detect bioluminescent cells or luminescence probes. To remedy this, Olympus has developed a completely new optical path including a tube lens with high exit NA and an extremely short distance between objective and detector, which allows highly sensitive imaging of bioluminescent cells.

Unrivalled resolution in luminescence microscopy

Due to the great increase in light output of the LV200 compared to a conventional microscope setup, the use of low-resolution photon counting systems or high pixel binning has become obsolete. Depending on the required time resolution, EM-CCD cameras or cooled, slow-scan CCD cameras can be used.

Wide range of magnification

The unique optical design of LV200 still uses standard, high-NA fluorescence objectives. This means that the whole range of magnifications and immersion techniques enabled by fluorescence objectives is available.

Integrated environmental control

Bioluminescence techniques are often used for prolonged live cell analyses, for example gene expression levels are monitored over a time period of one or two weeks. An inbuilt system for temperature control, humidity and gas flow (e.g. CO₂ and O₂ mix) helps to keep the cultured cells or tissue slices in a healthy condition throughout the entire observation.

Installation in normal lab environment

The unique LV200 light-tight enclosure shields the sample and optics from external light. Thanks to this technology, most applications do not require a darkroom, which increases work efficiency.

Integrated excitation and emission filter wheels

For a combined bioluminescence and fluorescence image, motorised filter wheels have been integrated in the LV200. The emission filters can also be used to discriminate between bioluminescence probes with separate emission spectra.

LV200 Bioluminescence System

Observation methodsLuminescence imaging, transmitted bright field, basic transmitted fluorescence
LV200 main bodyLight tight dark box
Manual objective lens focusing
Co-axial XY stage
Motorised exciting filter wheel with 3 positions for standard 25 mm optical filters
Motorised shutter integrated in filter wheel
Motorised emission filter wheel with 6 positions for standard 25 mm optical filters
Condenser for transmission bright field coupled to light guide
C-mount for camera
Tube lens optimised for luminescence imaging, 0.2 x magnification

Accessories and options

Illumination unitExternal halogen lamp housing coupled with light guide
ObjectivesIntegration of all standard size Olympus objectives possible
ControllerIX-UCB Controller for filter wheels, illumination and shutter
Hand switchHand switch for filter wheels, illumination and shutter
Environmental controlDouble-layered chamber type incubator for 35 mm dish including controller, stage heater, top cover heater, main body heater, flowmeter for 5% CO₂, 95% air
System tableca. 700(H) x 600(D) mm (needed if large size camera is used)
Motorised z-focus driveAdaptor for motorised z-focus, controller
ComputationImaging computer with at least: Intel Core2Duo, 3GHz, 2GB RAM, 160GB and 500GB hard disks, dual head video board, DVD RW, USB, serial, LAN, video, audio on board, keyboard (GB),optical mouse, MSWindows X
Device controllerController board for timing of experiments, mounted inside imaging computer, control CCD trigger, filter wheel, shutter and peripherals
MonitorComputer flat panel monitor 20" TFT
SoftwareAdvance imaging software xcellence for MSWindows. Graphical interface for experiment planning and execution ("Experiment Manager"). Structured database for multi-dimensional data handling. Simple measurement functions.
Camera optionsDepending on application and required sensitivity cooled CCD cameras, EM-CCD cameras or deep cooled slow scan CCD cameras can be used. (Please ask for camera / software compatibility)
Power consumptionMain unit 850 VA
Controller 840 VA
Installation spaceca. 1500(W) x 750(D) mm (differ with configuration)
Total mass weightca. 70 kg (differ with configuration)
  • video_preview

    LV200 application image - HeLa cells using GL3 luciferase

    Gene Expression and Promotor Analysis using Heat Shock Response.

    JPG [ 288 KB] – LV200 application image - HeLa cells using GL3 luciferase

    Gene Expression and Promotor Analysis using Heat Shock Response.

  • video_preview

    LV200 application image - NIH/3T3 cell

    NIH/3T3 cell transfected with a fusion of CLOCK gene PER2 and luciferase.

    JPG [ 81 KB] – LV200 application image - NIH/3T3 cell

    NIH/3T3 cell transfected with a fusion of CLOCK gene PER2 and luciferase.

  • video_preview

    LV200 application image - mouse NIH3T3 fibroblasts

    Mouse NIH3T3 fibroblasts, stably expressing the full-length circadian Dbp gene fused to luciferase (low-resolution display of the whole time series).

    JPG [ 209 KB] – LV200 application image - mouse NIH3T3 fibroblasts

    Mouse NIH3T3 fibroblasts, stably expressing the full-length circadian Dbp gene fused to luciferase (low-resolution display of the whole time series).

  • video_preview

    LV200 application image - luciferase, GFP and brightfield

    LV200 application image - luciferase, fluorescent signal (GFP) and brightfield image.

    JPG [ 221 KB] – LV200 application image - luciferase, GFP and brightfield

    LV200 application image - luciferase, fluorescent signal (GFP) and brightfield image.

  • video_preview

    LV200 application image - HeLa cells

    Luminescent signal from HeLa cells expressing firefly luciferase in peroxisomes.

    JPG [ 145 KB] – LV200 application image - HeLa cells

    Luminescent signal from HeLa cells expressing firefly luciferase in peroxisomes.

  • video_preview

    LV200 application image - CHO cells

    Ca2+ assay using CHO cells expressing Photina®

    JPG [ 222 KB] – LV200 application image - CHO cells

    Ca2+ assay using CHO cells expressing Photina®

  • video_preview

    LV200 application image - bilateral SCN

    High-magnification image of the bilateral SCN, with individual cells highlighted by green arrowheads, individual cell observation.

    JPG [ 83 KB] – LV200 application image - bilateral SCN

    High-magnification image of the bilateral SCN, with individual cells highlighted by green arrowheads, individual cell observation.

  • video_preview

    LV200 application image - LUC luciferase

    High-magnification image of the bilateral SCN, with individual cells highlighted by green arrowheads, variations from and similarities to the gross expression.

    JPG [ 81 KB] – LV200 application image - LUC luciferase

    High-magnification image of the bilateral SCN, with individual cells highlighted by green arrowheads, variations from and similarities to the gross expression.

  • video_preview

    LV200 application image

    High-magnification image of the bilateral SCN, with individual cells highlighted by green arrowheads, variations from and similarities to the gross expression.

    JPG [ 72 KB] – LV200 application image

    High-magnification image of the bilateral SCN, with individual cells highlighted by green arrowheads, variations from and similarities to the gross expression.

  • Play

    LV200 application video - DBP luciferase

    DBP luciferase
  • Play

    LV200 application video - gadi bmalluc transient

    Gadi bmalluc transient

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