Conventional microscope frames are optimised for transmission and fluorescence applications and are not sensitive enough to detect bioluminescent cells or luminescence probes. To remedy this, Olympus has developed a completely new optical path including a tube lens with high exit NA and an extremely short distance between objective and detector, which allows highly sensitive imaging of bioluminescent cells.
The LV200 has been carefully designed for long-duration cell bioluminescence microscopy and is compatible with a broad range of cameras and objectives. A completely new optical design dramatically increases sensitivity and enables the detailed study of photosensitive cells and luminescence probes at high magnification. An inbuilt system for temperature control, humidity and gas flow helps to keep the cultured cells or tissue slices in a healthy condition throughout the observation period and the unique light-tight enclosure shields the sample and optics from any external light.Add to
Due to the great increase in light output of the LV200 compared to a conventional microscope setup, the use of low-resolution photon counting systems or high pixel binning has become obsolete. Depending on the required time resolution, EM-CCD cameras or cooled, slow-scan CCD cameras can be used.
The unique optical design of LV200 still uses standard, high-NA fluorescence objectives. This means that the whole range of magnifications and immersion techniques enabled by fluorescence objectives is available.
Bioluminescence techniques are often used for prolonged live cell analyses, for example gene expression levels are monitored over a time period of one or two weeks. An inbuilt system for temperature control, humidity and gas flow (e.g. CO₂ and O₂ mix) helps to keep the cultured cells or tissue slices in a healthy condition throughout the entire observation.
The unique LV200 light-tight enclosure shields the sample and optics from external light. Thanks to this technology, most applications do not require a darkroom, which increases work efficiency.
For a combined bioluminescence and fluorescence image, motorised filter wheels have been integrated in the LV200. The emission filters can also be used to discriminate between bioluminescence probes with separate emission spectra.
LV200 Bioluminescence System
|Observation methods||Luminescence imaging, transmitted bright field, basic transmitted fluorescence|
|LV200 main body||Light tight dark box|
Manual objective lens focusing
Co-axial XY stage
Motorised exciting filter wheel with 3 positions for standard 25 mm optical filters
Motorised shutter integrated in filter wheel
Motorised emission filter wheel with 6 positions for standard 25 mm optical filters
Condenser for transmission bright field coupled to light guide
C-mount for camera
Tube lens optimised for luminescence imaging, 0.2 x magnification
Accessories and options
|Illumination unit||External halogen lamp housing coupled with light guide|
|Objectives||Integration of all standard size Olympus objectives possible|
|Controller||IX-UCB Controller for filter wheels, illumination and shutter|
|Hand switch||Hand switch for filter wheels, illumination and shutter|
|Environmental control||Double-layered chamber type incubator for 35 mm dish including controller, stage heater, top cover heater, main body heater, flowmeter for 5% CO₂, 95% air|
|System table||ca. 700(H) x 600(D) mm (needed if large size camera is used)|
|Motorised z-focus drive||Adaptor for motorised z-focus, controller|
|Computation||Imaging computer with at least: Intel Core2Duo, 3GHz, 2GB RAM, 160GB and 500GB hard disks, dual head video board, DVD RW, USB, serial, LAN, video, audio on board, keyboard (GB),optical mouse, MSWindows X|
|Device controller||Controller board for timing of experiments, mounted inside imaging computer, control CCD trigger, filter wheel, shutter and peripherals|
|Monitor||Computer flat panel monitor 20" TFT|
|Software||Advance imaging software xcellence for MSWindows. Graphical interface for experiment planning and execution ("Experiment Manager"). Structured database for multi-dimensional data handling. Simple measurement functions.|
|Camera options||Depending on application and required sensitivity cooled CCD cameras, EM-CCD cameras or deep cooled slow scan CCD cameras can be used. (Please ask for camera / software compatibility)|
|Power consumption||Main unit 850 VA|
Controller 840 VA
|Installation space||ca. 1500(W) x 750(D) mm (differ with configuration)|
|Total mass weight||ca. 70 kg (differ with configuration)|